The Saliva Multi-Drug Test is a one-step lateral flow chromatographic immunoassay based on the principle of competition for limited antibody binding sites between the drug in the sample and a drug-protein conjugate immobilized on a porous membrane support. During testing, saliva migrates to the testing area of the membrane by capillary action, mobilizing the colored antibody conjugates. The antibody conjugates then move along the membrane to the test area. In the absence of drug, or if the drug concentration in oral fluid is below the cutoff limit, the colored conjugates attach to the respective drug antigen immobilized in the test line region, forming a colored band (T line). If drug is present in oral fluid, the drug competes for limited antibody binding sites. If the drug concentration is at or above the cutoff limit, the drug will saturate all the binding sites of the antibody, preventing the attachment of the colored conjugates to the antigen in the test line area of the membrane.
This test is capable of detecting specific drugs and/or drug metabolites in human oral fluid at or above the cutoff concentrations indicated in the Intended Use section.
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